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Fig. 4 | Parasites & Vectors

Fig. 4

From: Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasis

Fig. 4

Multiplex PCR amplification of Leishmania genomic DNA from lesion samples using the newly developed primer pairs subjected to electrophoresis on a 1.0% agarose gel. Lane 1: amplicon of L. major MRHO/IR/75/ER reference strain; Lane 2: amplicon of a patient’s L. major isolate. Lane 3: amplicon of L. tropica MHOM/SU/74/K27 reference strain; Lane 4: amplicon of a patient’s L. tropica isolate; Lane 5: amplicon of L. infantum MCAN/IR/96/LON49 reference strain; Lane 6: amplicon of a patient’s L. infantum isolate. Lane 7: non-template negative control. Lane L: 100 bp DNA ladder

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