Fig. 4From: Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasisMultiplex PCR amplification of Leishmania genomic DNA from lesion samples using the newly developed primer pairs subjected to electrophoresis on a 1.0% agarose gel. Lane 1: amplicon of L. major MRHO/IR/75/ER reference strain; Lane 2: amplicon of a patient’s L. major isolate. Lane 3: amplicon of L. tropica MHOM/SU/74/K27 reference strain; Lane 4: amplicon of a patient’s L. tropica isolate; Lane 5: amplicon of L. infantum MCAN/IR/96/LON49 reference strain; Lane 6: amplicon of a patient’s L. infantum isolate. Lane 7: non-template negative control. Lane L: 100 bp DNA ladderBack to article page