Table 1 Details of primers used for mammalian DNA and B. bovis qPCR detection assays
Target gene | Primer/probe name | Sequence | Size (bp) | References |
|---|---|---|---|---|
18S | Reverse: S0933_BoR_18S | AGTCGTGCGTCATCGACAAA | 20 | Kim et al. [26] |
18S | Forward: S0934_BoF_18S | AGCAGGTTTCGCCTGTATAATG | 22 | |
18S | Probe (5′-FAM-3′): S0935_BoP | CCTTGTATGACCCTGTCGTACCGTTGG | 27 | |
Cytochrome b | Forward: S0936_bovisF160_Cytb | ATATGTTTGCATTTGCTG | 18 | Zhang et al. [27] |
Cytochrome b | Reverse: S0937_bovisR249_cytb | CTCCAAACCAATATGAAAG | 19 | |
Cytochrome b | Probe (5′-HEX-3′): S0938_bovis_cytb | CAAACCATAAAGTCATCGGTATATCCTAC | 29 | |
GAPDH | Forward: S0631_Dog_F | TCAACGGATTTGGCCGTATTGG | 22 | Nijhof et al. [25] |
GAPDH | Reverse: S0634_Dog_R | TGAAGGGGTCATTGATGGCG | 20 | |
GAPDH | Probe (5′-HEX-3′): S0632_Dog | CAGGGCTGCTTTTAACTCTGGCAAAGTGGA | 30 |