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Table 1 Mean concentration (ng/µl) and 260/280 purity ratios of DNA extracts obtained from phlebotomine sand flies according to different preservation methods and storage times. PCR success (positive/total and percentage) for the mitochondrial cytochrome c oxidase subunit 1 (cox1) and cacophony (CAC) genes is also shown

From: Evaluation of different storage times and preservation methods on phlebotomine sand fly DNA concentration and purity

Preservation method Storage time DNA concentration (mean ± SD) 260/280 ratio (mean ± SD) Success rate of PCR
CAC gene % cox1 gene %
70% ethanol at room temperature (G1) T1 4.5 ± 1.8 1.8 ± 0.4 10/10 100  10/10 100
T2 3.0 ± 0.9 2.0 ± 0.2 10/10 100 10/10 100
T3 4.9 ± 2.2 1.9 ± 0.2 10/10 100 10/10 100
T4 2.6 ± 1.4 1.9 ± 0.1 10/10 100 10/10 100
T5 3.7 ± 1.1 1.9 ± 0.2 10/10 100 10/10 100
95% ethanol at room temperature (G2) T1 8.2 ± 5.7 1.7 ± 0.5 9/10 90 10/10 100
T2 2.7 ± 0.6 1.9 ± 0.2 10/10 100 10/10 100
T3 5.2 ± 5.7 1.8 ± 0.2 10/10 100 10/10 100
T4 2.7 ± 0.5 2.0 ± 0.2 10/10 100 10/10 100
T5 3.6 ± 0.7 2.0 ± 0.3 10/10 100 10/10 100
70% ethanol at 8 °C (G3) T1 4.2 ± 2.7 1.8 ± 0.2 10/10 100 10/10 100
T2 2.3 ± 1.5 1.9 ± 0.2 10/10 100 10/10 100
T3 3.1 ± 0.9 1.9 ± 0.3 10/10 100 10/10 100
T4 2.7 ± 1.1 1.9 ± 0.2 10/10 100 10/10 100
T5 3.0 ± 0.7 2.1 ± 0.2 10/10 100 10/10 100
95% ethanol at 8 °C (G4) T1 4.9 ± 3.1 1.8 ± 0.4 10/10 100 10/10 100
T2 3.2 ± 0.5 1.8 ± 0.2 10/10 100 10/10 100
T3 3.8 ± 1.2 1.9 ± 0.2 10/10 100 10/10 100
T4 3.1 ± 1.2 1.9 ± 0.2 10/10 100 10/10 100
T5 3.3 ± 1.3 2.0 ± 0.3 10/10 100 10/10 100
Frozen dry at − 20 °C (G5) T1 7.7 ± 3.8 1.6 ± 0.3 10/10 100 10/10 100
T2 4.0 ± 1.8 1.8 ± 0.2 10/10 100 10/10 100
T3 4.5 ± 2.2 1.8 ± 0.2 10/10 100 10/10 100
T4 4.5 ± 2.4 1.9 ± 0.2 10/10 100 10/10 100
T5 4.3 ± 0.9 1.9 ± 0.2 10/10 100 10/10 100
  1. Abbreviations: T1, < 12 h; T2, 3 months; T3, 6 months; T4, 9 months; T5, 12 months