Induction of long-lived potential aestivation states in laboratory An. gambiae mosquitoes

Parasites & Vectors

Table 1 Conditions and survival characteristics for priming and adult rearing temperature conditions

Temp	Species	Light. hours	Primed^a	n	Mean	SE	Median	95% CI	Max
27	M	12	N	101	21.34	0.62	21	21–22	42
27	M	12	Y	101	19.28	0.69	19	19–20	45
27	M	8	N	25	33.20	2.09	36	29–41	49
27	M	8	Y	25	30.45	2.43	30	25–41	49
27	S	12	N	100	18.55	0.51	17	17–20	38
27	S	12	Y	100	22.50	0.97	21	20–23	51
27	S	8	N	25	27.74	2.71	28	20–41	56
27	S	8	Y	25	32.64	3.03	30	27–41	65
20	M	12	N	100	40.03	2.02	37	29–48	98
20	M	12	Y	100	44.80	1.85	48	41–54	84
20	M	8	N	101	46.84	1.95	48	42–54	100
20	M	8	Y	101	43.67	1.86	44	40–49	93
20	S	12	N	100	41.34	1.86	37	31–42	92
20	S	12	Y	100	46.48	1.86	49	42–55	84
20	S	8	N	100	44.02	1.69	45.5	41–50	97
20	S	8	Y	100	55.20	1.84	62	54–68	109

Notes: See also Additional file 2: Figure S2. Three replicates were performed for all groups (group size ~ 25, ~ 50, and ~ 25 per replicate) except for 27 °C and 8:16 L:D which was added during the final replicate (group size 25)
CI, confidence interval; Max, maximum; SE, standard error
^aPrimed mosquitoes kept in a 11:13 Light:Dark (L:D), 40–85% relative humidity (RH), 20–32 °C daily cycle (see Additional file 1: Figure S1) until their first blood meal at which they were moved into the listed temperature and light hour conditions. Non-priming conditions are standard 12:12 L:D, 85% RH, 27 °C

ISSN: 1756-3305