Fig. 3

Effects of inducible nitric oxide synthase (iNOS) inhibition by aminoguanidine (AG) on Trichobilharzia regenti infection in mice. No effect on parasite burden was observed 18 h post-infection (hpi) in the skin (a) and 3 days post-infection (dpi) in the spinal cord (b). However, moderately increased parasite burden was noticed in the spinal cord, but not cerebellum of AG-treated mice (red boxplots) 7 dpi in comparison with the control group treated by phosphate-buffered saline (PBS; blue boxplots) (c). The graphs a-c show pooled data from two experiments, each using five mice per group. Schistosomula isolated from AG-treated mice were shorter (d) and thinner (e) than those from control mice 3 dpi, but the opposite was observed 7 dpi. For each time point, 36–56 schistosomula fixed in hot water were measured. Data were evaluated by Mann-Whitney test (a) or two-way ANOVA followed by Šidák’s test (b–e); **P < 0.01, ***P < 0.001