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Fig. 1 | Parasites & Vectors

Fig. 1

From: Unveiling the immunomodulatory properties of Haemonchus contortus adhesion regulating molecule 1 interacting with goat T cells

Fig. 1

Molecular characterization of HcADRM1 derived from HcESPs. a Alignment of HcADRM1 amino acid sequences with other orthologues. The positions of ADRM1 family motifs are indicated above the multiple sequence alignment, containing zebrafish (XP_021325529.1), human (NP_001268366.1), mouse (NP_062796.2), C. elegans (NP_498387.2) and H. contortus (W6NB91) ADRM1 ortholog sequences retrieved from GenBank. An asterisk indicates the position with one completely conserved amino acid, while period denotes weakly conserved similarity within different groups and colon represents strongly similar conservation between groups. b Phylogenetic analysis of HcADRM1 with vertebrate and parasite orthologues. Evolutionary relationships of taxa were inferred using the Maximum Likelihood method with protein sequences including Mus musculus (NP_062796.2), Homo sapiens (XP_011526805.1), Danio rerio (XP_021325529.1), Toxocara canis (KHN87202.1), C. elegans (NP_498387.2), Dictyocaulus viviparus (KJH49054.1), T. circumcincta (PIO73930.1), H. contortus (W6NB91), Oesophagostomum dentatum (KHJ97330.1) and Ancylostoma caninum (RCN48176.1). Bootstrap support values are shown for each node. The scale-bar denotes the number of substitutions per site. c Acquisition of rHcADRM1 proteins and western blot analysis. Lanes M: protein standard ladder; Lane 1: rHcADRM1 expressed in the supernatant of cell lysates; Lane 2: SDS-PAGE analysis of purified rHcADRM1 protein; Lane 3: immunoblot analysis of rHcADRM1 using anti-H. contortus serum as primary antibody; Lane 4: immunoblot analysis of rHcADRM1 using normal goat serum (control) as primary antibody; Lane 5: immunoblot analysis of HcESPs using rat anti-rHcADRM1 IgG as primary antibody; Lane 6: immunoblot analysis of HcESPs using normal rat IgG (control) as primary antibody. d HcADRM1 expression in H. contortus life-cycle stages. Data are presented as the mean ± SD. e Immunolocalization of native HcADRM1 protein in male and female adults. The immunohistochemistry assays were performed using normal rat IgG (control) or rat anti-rHcADRM1 IgG as primary antibody. Cy3-coupled fluorescence (red), along with DAPI (blue), was identified for the investigation of HcADRM1 distribution. Scale-bars: e, 200 µm

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