Fig. 2

SEM micrographs showing enlarged images of sand fly SGs of P. sergenti. a and b show the marks of the epithelial cells (arrows) and c shows a shrunken surface of a flaccid SG lobe. d, e Histological sections of SGs, respectively, of Lu. longipalpis (d) and Lu. migonei (e). The secretory epithelium (ep) of Lu. migonei is in a straight line differing from Lu. longipalpis, which shows a single lucent cell (arrow) or a group of dark cells (asterisk) projecting into the saliva reservoir (Sr). f Sequential photomicrographs of SGs stained with the nuclear mark (DAPI) pictured at distinct focuses (from 16 images). All nuclei were marked over a transparent paper (as seen in the sketch on the right) and the cell number was counted. Scale-bars: a, b, c, 20 µm; d, e, 30 µm; f, 200 µm