Fig. 3

LSM pictures of lectins labeling the SG. a Con A lectin labeling of Lu. longipalpis (Lulo, a 3D image), P. duboscqi (Pdub) and Lu. migonei (Lumig). Note the presence of strong labeling in some secretory cells of Lu. migonei (arrows in Lumig). Scale-bars: Lulo, 40 µm; Pdub and Lumig, 50 µm. b HPA A lectin. The SG of P sergenti (Pserg) shows labeling in the basal lamina (arrows) and in the intercellular spaces of the secretory cells. In the SG of Lu. longipalpis (Lulo), the labeling is almost not visible (arrows). Scale-bars: Pserg, 50 µm; Lulo, 100 µm. c WGA lectin. The SGs show strong reactions in the intercellular spaces among the epithelial secretory cells in P. halepensis (Phal) and Lu. longipalpis (Lulo). Scale-bars: 100 µm. d RCA lectin. The SG is labeled in the basal lamina of P. halepensis (Phal) distinctly from Lu. migonei (Lumig), which presents a few fluorescent secretory cells. Scale-bars: Phal and Lumig, 100 µm. e UEA lectin. The SG of P. halepensis (Phal) is showing weak labeling in the basal lamina (arrows) differently from Lu. migonei (Lumig), which has a complete negative reaction. Scale-bars: 100 µm