Fig. 1

Promotion of TLR and NF-κB signaling molecules in HCT-8 cells by C. parvum. a Gel electrophoresis of qPCR products. b Expression of TLR2 and TLR4 at 4 and 12 hpi. Non-infected cells represent the control group (*P < 0.05 and ***P < 0.001 by t-test: test versus non-infected cells). c Western blots of TLR4, NF-κB, and IκBα protein at 4, 8 and 12 hpi; 0 hpi represents the control group