Fig. 2

Different expression profiles of the SjPrx-4 gene at various developmental stages of S. japonicum. a Transcription of the SjPrx-4 gene was detected by RT-PCR in the different developmental stages. Lane 1: male; Lane 2: female; Lane 3: eggs; Lane 4: sporocysts. Upper panel: transcription of SjPrx-4 gene; Lower panel: transcription of SjTPI gene as internal control. b Expression levels of the SjPrx-4 gene were determined by qRT-PCR. Data were analyzed according to 2^−ΔΔCq method using the TPI gene as the internal control for each sample. The fold changes of transcription levels in eggs and male and female worms were calculated relative to that of the sporocysts. Data are represented as the mean ± SE of three independent assays. *P < 0.05