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Table 3 Evaluation of primers and probes specificity

From: High sensitivity of one-step real-time reverse transcription quantitative PCR to detect low virus titers in large mosquito pools

Primer

Cq values

ZIKV

USUV

WNV

CHIKV

Negative

ZIKV

20.91

–

–

–

–

USUV

–

22.09

–

–

–

WNV

–

–

17.27

–

–

CHIKV

–

–

–

21.11

–

  1. Abbreviations: +, Cq < 36; –, Cq ≥ 36 or no signal
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Parasites & Vectors

ISSN: 1756-3305

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