Fig. 3

Conjugation of recombinant rhodanese protein from Haemonchus contortus (rHCRD) and goat peripheral blood mononuclear cells (PBMCs). Goat PBMCs were treated with rHCRD (40 μg/ml) or untreated at 37 °C for 1 h. After fixing, the cells were incubated with rat anti-rHCRD antibody followed by Cy3-labelled goat anti-rat IgG (red), and DAPI (blue) staining was used to visualize the nuclei. A confocal laser scanning microscope was used to visualize the binding of rHCRD and goat PBMCs. The overlapping blue and red channels were merged. Tests were independently performed in triplicate