Table 2 Primer sequences for the amplification of T. equi and/or B. caballi
Organism-s | Target gene | Assay | Product size (bp) | Primer | Sequence 5′–3′ | Annealing temperature (°C) | Reference |
|---|---|---|---|---|---|---|---|
T. equi | 18S rRNA | PCR | 435 | P1 | TCGAAGACGATCAGATACCGTCG | 60 | [14] |
P2 | TGCCTTAAACTTCCTTGCGAT | ||||||
B. caballi | BC48 | Nested PCR | 454 | P3 | ACGAATTCCCACAACAGCCGTGTT | 55 | [15] |
P4 | ACGAATTCGTAAAGCGTGGCCATG | ||||||
P5 | GGGCGACGTGACTAAGACCTTATT | ||||||
P6 | GTTCTCAATGTCAGTAGCATCCGC | ||||||
T. equi/B. caballi | 18S rRNA | Nested PCR | ~ 1400 | P7 | GAAAYTGCGAATGGCTCATTAM | 57 | [13] |
P8 | CACCGGATCACTCGATCGGTAGG | ||||||
P9 | GGATAACCGTGSTAATTSTAGGGC | ||||||
P10 | GTGTGTACAAAGGGCAGGGACG |