Fig. 6

Immunohistochemical staining with primary anti-TLR2 (a–h) and anti-TLR4 antibodies (i–p) in the heart of immunocompetent and immunosuppressed mice from control group (0 dpi) and at 8, 16 and 24 dpi. Magnification 100×. In the immunocompetent and immunosuppressed control mice, only some cardiomyocytes showed TLR2 expression (a, e). At the beginning of infection in immunocompetent mice, TLR2 expression was observed in most cardiomyocytes (b, c). The lowest immunohistochemical reaction in the heart of immunocompetent mice was found at 24 dpi (d). In the immunosuppressed Acanthamoeba spp.-infected mice, the highest intensity of TLR2 expression was noted at 8 and 16 dpi (f, g). In the 24 dpi group of immunosuppressed mice, TLR2 was expressed only in some cardiomyocytes (h). In the heart of immunocompetent Acanthamoeba spp.-infected mice, increased TLR4 expression at 8 dpi was observed (j), while at 16 dpi, decreased number of TLR4-positive cells was found (k). In the immunosuppressed Acanthamoeba spp.-infected mice, the highest TLR4 expression was observed in the hearts of mice at 8 and 16 dpi (n, o), while at 24 dpi there was a decrease in TLR4 expression (p). dpi days post-Acanthamoeba spp. infection, IC immunocompetent mice, IS immunosuppressed mice