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Table 2 Evaluation of the specificity of bCUBE-based qRT-PCR for DENV2 and ZIKV detection

From: Field-deployable molecular diagnostic platform for arbovirus detection in Aedes aegypti

Family

Genus

Species

Strain

BEI No

RNA concentration (ng/µl)

No. of positives for ZIKV assay

No. of positives for DENV2 assay

Flaviviridae

Flavivirus

Japanese encephalitis virus

India

NR-9592

0.1013

0/3

0/3

West Nile virus

CO 1862

NR-50434

0.10

0/3

0/3

Yellow fever virus

17D

NR-2869

0.120

0/3

0/3

Togaviridae

Alphavirus

Chikungunya virus

St Martin 2013

NR-50130

0.167

0/3

0/3

  1. Notes: A cross-reactivity panel of frequently co-circulating viruses was used to evaluate the specificity of bCUBE-based qRT-PCR for DENV2 and ZIKV. Arboviral RNA samples were obtained from BEI Resources. RNA concentration (ng/µl) and number of positively detected samples from the ZIKV and DENV2 assay are listed