Pathogen | Amplified gene | Primer sequences (5’–3’) | Fragment size (bp) | Annealing T (℃) | References |
---|---|---|---|---|---|
Anaplasma spp. | GroEL | GroEL‐F2: ATG(GT)CAAATACGGT(AT)GTCACGG | 855 | 62 | This study |
GroEL‐R8: TCRCCAAGCATRTCYTTTCTTC | |||||
Anaplasma/ Ehrlichia spp. | 16S rRNA | FD1: AGAGTTTTGATCCTGGCTCAG* | 426 | 55 | [25] |
EHR1: TAGCACTCATCGTTTACAGC | |||||
GA1UR: GAGTTTGCCGGGACTTCTTCT | |||||
Babesia spp. | 18S rRNA | BJ1: GTCTTGTAATTGGAATGATGG | 400–500 | 55 | [26] |
BN2: TAGTTTATGGTTAGGACTACG | |||||
Theileria spp. | 18S rRNA | THfor: TGACACAGGGAGGTAGTGA | 500 | 65 | [27] |
THrev: TCAGCCTTGCGACCATACT | |||||
A. marginale | MSP1α 1st PCR MSP1α 2nd PCR | 1733F: TGTGCTTATGGCAGACATTTCC 3134R1: TCACGGTCAAAACCTTTGCTTACC | 800–1000 | 55 | [9] |
1733F: TGTGCTTATGGCAGACATTTCC 2957R2: AAACCTTGTAGCCCCAACTTATCC | 60 |