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Table 1 Primers used for confirmation of co-infections and identification of Anaplasma marginale genotypes

From: Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt

Pathogen

Amplified gene

Primer sequences (5’–3’)

Fragment size (bp)

Annealing T (℃)

References

Anaplasma spp.

GroEL

GroEL‐F2: ATG(GT)CAAATACGGT(AT)GTCACGG

855

62

This study

GroEL‐R8: TCRCCAAGCATRTCYTTTCTTC

Anaplasma/ Ehrlichia spp.

16S rRNA

FD1: AGAGTTTTGATCCTGGCTCAG*

426

55

[25]

EHR1: TAGCACTCATCGTTTACAGC

GA1UR: GAGTTTGCCGGGACTTCTTCT

Babesia spp.

18S rRNA

BJ1: GTCTTGTAATTGGAATGATGG

400–500

55

[26]

BN2: TAGTTTATGGTTAGGACTACG

Theileria spp.

18S rRNA

THfor: TGACACAGGGAGGTAGTGA

500

65

[27]

THrev: TCAGCCTTGCGACCATACT

A. marginale

MSP1α 1st PCR

MSP1α 2nd PCR

1733F: TGTGCTTATGGCAGACATTTCC

3134R1: TCACGGTCAAAACCTTTGCTTACC

800–1000

55

[9]

1733F: TGTGCTTATGGCAGACATTTCC

2957R2: AAACCTTGTAGCCCCAACTTATCC

60

  1. Abbreviation: T, temperature