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Table 3 Evaluation of diagnostic assays for Anaplasma marginale against qPCR as a golden standard test

From: Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt

Species Diagnostic tool No. positive/No. total No. negative/No. total qPCR assay Parameter
TPd TNe FPf FNg Sensitivity Specificity PPVh NPVi CPVj
Cattle Blood smear 50/309 259/309 50 98 0 161 23.7 100 100 37.9 47.9
ELISAa 103/188 85/188 92 39 11 46 66.7 78.0 89.3 45.9 69.7
RLBb 155/309 154/309 128 83 15 83 60.7 84.7 89.5 50.0 68.3
Buffaloes Blood smear 2/85 83/85 2 60 0 23 8.0 100 100 72.3 72.9
RLBb 36/85 49/85 16 40 20 9 64.0 66.7 44.4 81.6 65.9
  1. Abbreviations: ELISA, enzyme-linked immunosorbent assay; RLB, reverse line blot; qPCR, real time PCR; TP, true positive; TN, true negative; FP, false positive; FN, false negative; PPV, positive predictive value; NPV, negative predictive value; CPV, combined predictive value (CPV)