Table 1 PCR systems used in this study and their sources
From: Tabanids as possible pathogen vectors in Senegal (West Africa)
Targeted organism | Target gene | Name | Primers (5′–3′) and probe | Tm* (°C) | Reference |
|---|---|---|---|---|---|
Tabanidae | cox1 | LCO1490 | GGTCAACAAATCATAAAGATATTGG | 50 | [49] |
HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | ||||
Hosts | 16S | 16SA-2290 | CGCCTGTTTACCAAAAACAT | 50 | [50] |
16SB-2860 | CCGGTCTGAACTCAGATC ACGT | ||||
Leishmania donovani complex | kDNA | RV1 | CTTTTCTGGTCCTCCGGGTAGG | 60 | [51] |
RV2 | CCACCCGGCCCTATTTTACACCAA | ||||
Probe. Leish* | FAM-TTTTCGCAGAACGCCCCTACCCGC-TAMRA | ||||
Leishmania spp. | ITS1 | rDNA-10 F | CAATACAGGTGATCGGACAGG | 55 | [52] |
rDNA-14 R | CACGGGGATGACACAATAGAG | ||||
Piroplasmida | 5.8S | 5.8S-F5 | AYYKTYAGCGRTGGATGTC | 60 | [53] |
5.8S-R | TCGCAGRAGTCTKCAAGTC | ||||
5.8S-S* | FAM-TTYGCTGCGTCCTTCATCGTTGT-MGB | ||||
Trypanosoma spp. | 5.8S | S. 5.8 S Tryp sp. | FAM-GTTGAAGAACGCAGCAAAGIGCGAT-TAMRA | 60 | [37] |
F. 5.8 S Tryp sp. | CAACGTGTCGCGATGGATGA | ||||
R. 5.8 S Tryp sp. | ATTCTGCAATTGATACCACTTATC | ||||
28S | F2 28S | ACCAAGGAGTCAAACAGACG | 58 | ||
R1 28S | GACGCCACATATCCCTAAG | ||||
ITS1 | ITS1-CF | CCGGAAGTTCACCGATATTG | 58 | [54] | |
ITS1-BR | TTGCTGCGTTCTTCAACGAA | ||||
Kinetoplastida spp. | 28S | F2 28S | ACCAAGGAGTCAAACAGACG | 58 | [55] |
R1 28S | GACGCCACATATCCCTAAG | ||||
18S | F 720 | GTTAAAGGGTTCGTAGTTGAA | 50 | This study | |
R1495 | GACTACAATGGTCTCTAATCA | ||||
Coxiella burnetii | IS1111 | F | CAAGAAACGTATCGCTGTGGC | 60 | [56] |
R | CACAGAGCCACCGTATGAATC | ||||
S | FAM- CCGAGTTCGAAACAATGAGGGCTG-TAMRA | ||||
Anaplasmataceae | 23S | TtAna-F | TGACAGCGTACCTTTTGCAT | 60 | [57] |
TtAna-R | GTAACAGGTTCGGTCCTCCA | ||||
TtAna-S* | FAM-CTTGGTTTCGGGTCTAATCC-TAMRA | ||||
Borrelia spp. | 16S | Bor_16S_3F | AGCCTTTAAAGCTTCGCTTGTAG | 60 | [58] |
Bor_16S_3R | GCCTCCCGTAGGAGTCTGG | ||||
Bor_16S_3P | FAM- CCGGCCTGAGAGGGTGAACGG-TAMRA | ||||
Ricketssia spp. | Citrate synthase (gltA) | RKNDO3_F | GTGAATGAAAGATTACACTATTTAT | 60 | [46] |
RKNDO3_R | GTATCTTAGCAATCATTCTAATAGC | ||||
RKNDO3_S | FAM-CTATTATGCTTGCGGCTGTCGGTTC-TAMRA | ||||
gltA (First PCR) | CS2D | ATGACCAATGAAAATAATAAT | 54 | [59]Â | |
CSEndR | CTTATACTCTCTATGTACA | ||||
gltA (nested PCR) | 409D | CCTATGGCTATTATGCTTGC | 54 | ||
1258R | ATTGCAAAAAGTACAGTGAACA | ||||
Mansonella spp. | ITS | Manso_ITS_F | CCTGCGGAAGGATCATTAAC | 60 | [20] |
Manso_ITS_R | ATCGACGGTTTAGGCGATAA | ||||
Manso_ITS_P | FAM- CGGTGATATTCGTTGGTGTCT-TAMRA | ||||
Filariae | 18S | Fwd.18S.631 | TCGTCATTGCTGCGGTTAAA | 54 | [60] |
Rwd.18S.1825r | GGTTCAAGCCACTGCGATTAA |