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Fig. 2 | Parasites & Vectors

Fig. 2

From: Cellular and molecular changes and immune response in the intestinal mucosa during Trichinella spiralis early infection in rats

Fig. 2

Kinetics of the appearance of innate effector cells in the small intestine during early T. spiralis infection. Goblet cells (a) were counted in the intestine epithelium using Alcian blue-PAS/Gill’s hematoxylin staining while eosinophils (b) and mast cells (c) were counted in the intestinal lamina propria using the Luna method and Alcian blue/saphranine stain, respectively. Cells were counted in non-infected and different dpi in tissue sections at 400× magnification per 15 villous crypt units (VCU) by two independent observers and results are expressed as mean of cell numbers/VCU ± SD from 5 rats/dpi. Data were analysed by adjusting to the negative binomial distribution followed by the Tukey’s multiple comparisons test (MASS and multcomp packages, respectively). Asterisks (*) indicate significant differences between infected and non-infected animals: *P < 0.05, **P < 0.001. Numerals (#) above bars indicate significant differences between days: #P < 0.05, ##P < 0.01; ###P < 0.001

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