Fig. 5

Kinetics of specific Igs secreting cells (Ig SCs) in the small intestine during early T. spiralis infection. Ig SCs were determined by ELISPOT assay in Payer’s patches (PP), mesenteric lymph nodes (MLN) and lamina propria (LP). Results are expressed as Ig SCs/10⁶ leucocytes mean ± SD obtained from 3 independent experiments performed with groups of 5 rats/dpi and 5 control rats. Data were analyzed by two-way ANOVA followed by the Tukey’s multiple comparisons test (nlme and multcomp packages, respectively). Asterisks (*) indicate significant differences between infected and non-infected animals: * P < 0.05, **P < 0.01, *** P < 0.001. Numerals (#) above bars indicate significant differences between days: ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001