Fig. 9

SEM analysis of cuticle thickness. a Schematic diagram of the experimental time course for siRNA microinjection and SEM. b SEM showing a front view of a sectioned leg of the siNC group and the siFTZ-F1 group. c Column bar graph (vertical) of the entire cuticle thickness. 16 points of measurement were used per individual, allowing for calculation of mean cuticle thickness. Results are shown as the mean ± SD. n = the number of measures carried out on each batch of 5 mosquitoes (siNC & siFTZ-F1). *P ≤ 0.05