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Fig. 2 | Parasites & Vectors

Fig. 2

From: Diminished adherence of Biomphalaria glabrata embryonic cell line to sporocysts of Schistosoma mansoni following programmed knockout of the allograft inflammatory factor

Fig. 2

Establishment of BgAIF-knockout lines of Bge cells. a Representative examples of frequent gene insertions-deletions (1–2 bp insertions and 8–30 bp deletions, straddling the programmed CRISPR/Cas9-induced double-stranded break in exon 4, as determined by ICE software-based analysis. b TIDE algorithm-based violin plot of insertion-deletion percentages (% INDEL) computed using the amplicon sequence traces from the 12 biological replicates of pCas-BgAIF-transfected Bge cell populations. c Reduction of BgAIF transcription by about 50% following programmed genome editing of Bge cells (∆BgAIF-Bge) in comparison to control Bge cells. Mean transcript reduction, 49.55 ± 20.22% (SD), P ≤ 0.0001**** (n = 12) (unpaired Student’s t-test)

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