Table 2 Primer sequences and annealing temperatures used to detect Rickettsia spp.
From: Molecular detection of Rickettsia in fleas from micromammals in Chile
Target gene | Primer name | Nucleotide sequence (5’–3’) | Annealing T (°C) | Product length (bp) |
|---|---|---|---|---|
ProgltA (401) | CS-78_F | GCAAGTATCGGTGAGGATGTAAT | 48a | 401 |
CS-323_R | GCTTCCTTAAAATTCAATAAATCAGGAT | |||
gltA (830) | CS-239_F | GCTCTTCTCATCCTATGGCTATTAT | 48a | 830 |
CS-1069_R | CAGGGTCTTCGTGCATTTCTT | |||
rpoB (395) | RirpoB_F | CCGACTCATTACGGTCGCATTTGT | 55.5 | 395 |
RirpoB_R | CCCATCAAAGCACGGTTAGCATCA | |||
sca5 (862) | 120.M59F | CCGCAGGGTTGGTAACTGC | 50b | 862 |
120.807R | CCTTTTAGATTACCGCCTAA | |||