Table 6 Similarity percentage for obtained sequences with BLAST analyses
From: Molecular detection of Rickettsia in fleas from micromammals in Chile
Flea collection | Identification by gene sequence (% similarity with the corresponding sequence on GenBank) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
gltA 401 bp | gltA 830 bp | rpoB 395 bp | sca5 862 bp | ||||||||
Flea species | Total sequences amplified per flea | Host | Collection site | Rickettsia spp. | No. positive | Rickettsia spp. | No. positive | Rickettsia spp. | No. positive | Rickettsia spp. | No. positive |
Chiliopsylla allophyla | 2 | Abrothrix hirta | Nonguén NR | Rickettsia sp. Gr15 (99%; KP675966) | 2 | “Candidatus Rickettsia senegalensis” (99%; KU499847) | 2 | Rickettsia sp. (100%; JF966777) | 2 | Rickettsia felis (94%; GQ385243) | 2 |
Ctenoparia inopinata | 1 | Abrothrix hirta | Los Queules NR | Rickettsia sp. Gr15 (97%; KP675966) | 1 | Rickettsia sp. (99%; KY799066) | 1 | Rickettsia sp. (97%; JF966777) | 1 | Rickettsia felis (94%; GQ385243) | 1 |
Delostichus phyllotis | 1 | Octodon degus | La Campana NP | Rickettsia sp. (100%; KY705378) | 1 | na | na | ||||
Leptopsylla segnis | 1 | Rattus rattus | Lolol | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | |||
Neotyphloceras chilensis | 4 | Phyllotis darwini | Las Chinchillas NR | Rickettsia sp. MEAM1 (99%; CP016305) | 4 | Rickettsia sp. (97%; KF646706) | 4 | Rickettsia sp. MEAM1 (97–98%; CP016305) | 4 | na | |
2 | Abrothrix olivacea | Las Chinchillas NR | Rickettsia sp. MEAM1 (98–99%; CP016305) | 2 | Rickettsia sp. (97%; KF646706) | 2 | Rickettsia sp. MEAM1 (98%; CP016305) | 2 | na | ||
1 | Abrothrix olivacea | Fray Jorge NP | Rickettsia sp. MEAM1 (99%; CP016305) | 1 | Rickettsia sp. (97%; U76908) | 1 | Rickettsia sp. MEAM1 (98%; CP016305) | 1 | na | ||
3 | Abrothrix olivacea | Canela Baja | Rickettsia sp. MEAM1 (99%; CP016305) | 2 | Rickettsia sp. (97%; U76908) | 3 | Rickettsia sp. MEAM1 (98%; CP016305) | 3 | na | ||
1 | Octodon degus | Canela Baja | Rickettsia sp. MEAM1 (99%; CP016305) | 1 | Rickettsia sp. (97%; U76908) | 1 | Rickettsia sp. MEAM1 (98%; CP016305) | 1 | na | ||
2 | Phyllotis darwini | Canela Baja | Rickettsia sp. MEAM1 (99%; CP016305) | 2 | Rickettsia sp. (97%; U76908) | 2 | Rickettsia sp. MEAM1 (98-100%; CP016305) | 2 | na | ||
1 | Thylamys elegans | Canela Baja | Rickettsia sp. MEAM1 (99%; CP016305) | 1 | Rickettsia sp. (97%; U76908) | 1 | Rickettsia sp. MEAM1 (98%; CP016305) | 1 | na | ||
Neotyphloceras crassispina | 1 | Oligoryzomys longicaudatus | Laguna Torca NR | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | |||
1 | Oligoryzomys longicaudatus | Cobquecura | na | na | Rickettsia sp. (94%; KX300203) | 1 | na | ||||
1 | Abrothrix hirta | Cobquecura | na | na | Rickettsia sp. (94%; KX300203) | 1 | na | ||||
Neotyphloceras pardinasi | 2 | Abrothrix hirta | Cobquecura | na | na | Rickettsia sp. (94–96%; KX300157) | 2 | na | |||
1 | Abrothrix olivacea | Cobquecura | na | na | Rickettsia sp. (94%; KX300157) | 1 | na | ||||
1 | Rattus rattus | La Campana NP | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | ||||
1 | Rattus rattus | Quirihue | na | na | Rickettsia sp. (94%; KX300203) | 1 | na | ||||
2 | Rattus rattus | Laguna Torca NR | Rickettsia sp. (99–100%; KY705378) | 2 | na | na | |||||
1 | Rattus rattus | Canela Baja | Rickettsia sp. (100%; KY705378) | 1 | Rickettsia sp. (97%; U76908) | 1 | Rickettsia sp. MEAM1 (93%; CP016305) | 1 | na | ||
Neotyphloceras spp. | 1 | Oligoryzomys longicaudatus | Cobquecura | na | na | Rickettsia sp. (95%; KX300157) | 1 | na | |||
1 | Abrothrix hirta | Laguna Torca NR | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | ||||
3 | Abrothrix olivacea | Laguna Torca NR | Rickettsia sp. (100%; KY705378) | 3 | na | na | na | ||||
1 | Oligoryzomys longicaudatus | Laguna Torca NR | Rickettsia sp. (99%; KY705378) | 1 | na | na | na | ||||
2 | Thylamys elegans | Laguna Torca NR | Rickettsia sp. (100%; KY705378) | 2 | na | na | na | ||||
2 | Abrothrix olivacea | Canela Baja | Rickettsia sp. MEAM1 (99%; CP016305) | 2 | Rickettsia sp. (97%; U76908) | 2 | Rickettsia sp. MEAM1 (98%; CP016305) | 2 | na | ||
1 | Abrothrix olivacea | Fray Jorge NP | Rickettsia sp. MEAM1 (99%; CP016305) | Rickettsia sp. (97%; U76908) | 1 | na | na | ||||
1 | Abrothrix hirta | Los Queules NR | Rickettsia sp. (97%; U59712) | 1 | na | Rickettsia sp. (91%; JF966777) | 1 | na | |||
1 | Abrothrix olivacea | Los Queules NR | na | na | Rickettsia sp. (94%; KX300157) | 1 | na | ||||
1 | Thylamys elegans | Fray Jorge NP | na | Rickettsia sp. (97%; U76908) | 1 | na | na | ||||
Nosopsyllus fasciatus | 2 | Rattus rattus | Lolol | Rickettsia sp. (100%; KY705378) | 2 | na | na | na | |||
1 | Rattus rattus | Santa Cruz | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | ||||
Plocopsylla spp. | 1 | Rattus rattus | La Campana NP | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | |||
1 | Rattus rattus | Til Til | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | ||||
Sphinctopsylla ares | 6 | Abrothrix hirta | Los Queules NR | Rickettsia sp. (97%; U59712) | 6 | Rickettsia sp. (98%; AJ269522) | 5 | Rickettsia sp. (91%; JF966777) | 5 | na | |
2 | Abrothrix hirta | Cobquecura | Rickettsia sp. (98%; U59712) | 2 | Rickettsia sp. (98%; AJ269522) | 1 | Rickettsia sp. (91%; JF966777) | 2 | na | ||
1 | Abrothrix hirta | Coyhaique NR | Rickettsia sp. (98%; U59712) | 1 | Rickettsia sp. (98%; AJ269522) | 1 | Rickettsia sp. (93%; JF966777) | 1 | na | ||
3 | Abrothrix olivacea | Coyhaique NR | Rickettsia sp. (97%; U59712) | 3 | Rickettsia sp. (98%; AJ269522) | 3 | Rickettsia sp. (93%; JF966777) | 3 | na | ||
2 | Abrothrix olivacea | Los Queules NR | Rickettsia sp. (98%; U59712) | 1 | Rickettsia sp. (96%; KY799066) | 2 | Rickettsia sp. (91–93%; JF966777) | 3 | Rickettsia hoogstraalii (88%; EF629536) | 2 | |
2 | Abrothrix olivacea | Cobquecura | na | Rickettsia sp. (95%; KX300157) | 2 | na | |||||
1 | Abrothrix olivacea | Cobquecura | Rickettsia sp. (93%; KY433588) | 1 | na | Rickettsia sp. (95%; KX300157) | 1 | na | |||
1 | Oligoryzomys longicaudatus | Cobquecura | na | na | Rickettsia sp. (95%; KX300157) | 1 | na | ||||
2 | Rattus rattus | Laguna Torca NR | Rickettsia sp. (100%; KY705378) | 2 | na | na | na | ||||
1 | Thylamys elegans | Laguna Torca NR | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | ||||
Tetrapsyllus rhombus | 1 | Abrothrix hirta | Los Queules NR | Rickettsia sp. (98%; U59712) | 1 | Rickettsia sp. (98%; AJ269522) | 1 | Rickettsia sp. (91%; JF966777) | 1 | na | |
1 | Abrothrix hirta | Cobquecura | Rickettsia sp. (97%; U59712) | 1 | Rickettsia sp. (98%; AJ269522) | 1 | Rickettsia sp. (91%; JF966777) | 1 | na | ||
3 | Abrothrix olivacea | Los Queules NR | Rickettsia sp. (97%; U59712) | 3 | Rickettsia sp. (98%; AJ269522) | 3 | Rickettsia sp. (91%; JF966777) | 3 | na | ||
1 | Abrothrix olivacea | Los Queules NR | na | na | Rickettsia sp. (94%; KX300203) | 1 | na | ||||
1 | Abrothrix olivacea | Coyhaique NR | Rickettsia sp. (97%; U59712) | 1 | Rickettsia sp. (98%; AJ269522) | 1 | Rickettsia sp. (93%; JF966777) | 1 | na | ||
1 | Rattus rattus | La Campana NP | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | ||||
Tetrapsyllus tantillus | 4 | Abrothrix olivacea | Lolol | Rickettsia sp. (100%; KY705378) | 4 | na | na | na | |||
1 | Abrothrix olivacea | Til Til | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | ||||
1 | Octodon degus | La Campana NP | Rickettsia sp. (100%; KY705378) | 1 | na | na | na | ||||