Evidence of vector borne transmission of Salmonella enterica enterica serovar Gallinarum and fowl typhoid disease mediated by the poultry red mite, Dermanyssus gallinae (De Geer, 1778)

Table 2 Detection and quantification of Salmonella enterica enterica serovar Gallinarum from mites

Mite aliquot	Isolator	Day of collection	Formaldehyde-washed	snPCR result	SG cells/mite
AD9	A	D9	No	+	64.50
BD9	B	D9	No	+	16.75
AD10	A	D10	No	+	1.42
BD10	B	D10	No	+	nd^a
AD11	A	D11	No	+	0.88
BD11	B	D11	No	−	–
AD36	A	D36	No	+	28.71
BD36	B	D36	No	+	14.29
CD36-1	C	D36	No	+	389.27
CD36-2	C	D36	No	+	5.25
CD36-3	C	D36	No	+	65.71
CD36-4	C	D36	No	+	629.05
DD36-1	D	D36	No	+	69.11
DD36-2	D	D36	No	+	5.96
DD36-3	D	D36	No	+	16.50
CD36-5	C	D36	Yes	+	274.22
CD36-6	C	D36	Yes	+	6.05
CD36-7	C	D36	Yes	+	64.15
CD36-8	C	D36	Yes	+	958.73
CD36-9	C	D36	Yes	+	0.03
CD36-10	C	D36	Yes	+	16.99
DD36-4	D	D36	Yes	+	nd^a
DD36-5	D	D36	Yes	+	nd^a
DD36-6	D	D36	Yes	+	0.14
DD36-7	D	D36	Yes	+	10.34

^and: non-detectable. A late amplification curve is observed in qPCR, with putative quantification < 0.01 SG cells/mite
Abbreviations: SG, serovar Gallinarum; +, SG detected; −, SG not detected

ISSN: 1756-3305