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Table 2 Detection and quantification of Salmonella enterica enterica serovar Gallinarum from mites

From: Evidence of vector borne transmission of Salmonella enterica enterica serovar Gallinarum and fowl typhoid disease mediated by the poultry red mite, Dermanyssus gallinae (De Geer, 1778)

Mite aliquot Isolator Day of collection Formaldehyde-washed snPCR result SG cells/mite
AD9 A D9 No + 64.50
BD9 B D9 No + 16.75
AD10 A D10 No + 1.42
BD10 B D10 No + nda
AD11 A D11 No + 0.88
BD11 B D11 No
AD36 A D36 No + 28.71
BD36 B D36 No + 14.29
CD36-1 C D36 No + 389.27
CD36-2 C D36 No + 5.25
CD36-3 C D36 No + 65.71
CD36-4 C D36 No + 629.05
DD36-1 D D36 No + 69.11
DD36-2 D D36 No + 5.96
DD36-3 D D36 No + 16.50
CD36-5 C D36 Yes + 274.22
CD36-6 C D36 Yes + 6.05
CD36-7 C D36 Yes + 64.15
CD36-8 C D36 Yes + 958.73
CD36-9 C D36 Yes + 0.03
CD36-10 C D36 Yes + 16.99
DD36-4 D D36 Yes + nda
DD36-5 D D36 Yes + nda
DD36-6 D D36 Yes + 0.14
DD36-7 D D36 Yes + 10.34
  1. and: non-detectable. A late amplification curve is observed in qPCR, with putative quantification < 0.01 SG cells/mite
  2. Abbreviations: SG, serovar Gallinarum; +, SG detected; −, SG not detected