Fig. 2

Wright’s staining of intracellular amastigotes in cells after treated with SSG and inhibition rates of SSG on intracellular amastigotes. a Intracellular amastigotes representing reference sensitive 9044 and DD8 strains, and HCZ isolate of L. donovani after 24 and 48 h of incubation with PBS (control) and SSG. b Inhibition rates of SSG on the number of intracellular amastigotes in macrophages after 24 and 48 h of treatment. At least three independent experiments were carried out and figures shown are representative of these experiments. The black arrows indicated intracellular amastigotes. ***P < 0.001, ****P < 0.0001. Scale-bars: 20 µm