Table 1 Primer sequences used for diagnostic testing of Wolbachia
From: Wolbachia in mosquitoes from the Central Valley of California, USA
Test | Gene target | PCR product (bp) | Primer name | Sequence (5ʹ-3ʹ) | References |
|---|---|---|---|---|---|
Wolbachia presence | 16S rRNA | 438 | W16S-F | CATACCTATTCGAAGGGATAG | |
W16S-R | TTGCGGGACTTAACCCAACA | ||||
wsp (General) | 185 | wsp-F | GCATTTGGTTAYAAAATGGACGA | [97] | |
wsp-R | GGAGTGATAGGCATATCTTCAAT | ||||
Wolbachia density | RpS3 | 70 | RpS3-F | AGCGTGCCAAGTCGATGAG | [98] |
RpS3-R | ACGTACTCGTTGCACGGATCTC | ||||
Supergroup A/B identification | wsp (Supergroup A) | 556 | 136F | TGAAATTTTACCTCTTTT | [65] |
691R | AAAAATTAAACGCTACTCCA | ||||
wsp (Supergroup B) | 442 | 81F | TGGTCCAATAAGTGATGAAGAAAC | Â | |
522R | ACCAGCTTTTGCTTGATA | ||||
Multilocus sequence typing | gatB | 396 | gatB_F1 | GAKTTAAAYCGYGCAGGBGTT | [57] |
gatB_R1 | TGGYAAYTCRGGYAAAGATGA | Â | |||
coxA | 402 | coxA_F1 | TTGGRGCRATYAACTTTATAG | Â | |
coxA_R1 | CTAAAGACTTTKACRCCAGT | Â | |||
hcpA | 444 | hcpA_F1 | GAAATARCAGTTGCTGCAAA | Â | |
hcpA_R1 | GAAAGTYRAGCAAGYTCTG | Â | |||
ftsZ | 435 | ftsZ_F1 | ATYATGGARCATATAAARGATAG | Â | |
ftsZ_R1 | TCRAGYAATGGATTRGATAT | Â | |||
fbpA | 429 | fbpA_F1 | GCTGCTCCRCTTGGYWTGAT | Â | |
fbpA_R1 | CCRCCAGARAAAAYYACTATTC | Â |