Table 2 Identification of vertebrate hosts of the Simulium turgaicum complex based on the host cytb gene in ingested blood meals by multiplex and conventional PCR assays in the Aras River Basin, Iran
Village | Ecotope (No. of blood-fed specimens) | No. of blood-fed specimens in each village | Multiplex PCR | Host-specific PCR | |||||
|---|---|---|---|---|---|---|---|---|---|
n | Hosts | n | Hosts | ||||||
B | H | C | D | ||||||
Qarloujeh | Humans/animals outdoors (n = 23); irrigation canals (n = 14); lands along the river (n =17); rice and alfalfa fields (n = 19) | 73 | 18 | DH (n = 1); CD (n = 2); BD (n = 2); CH (n = 9); BH (n = 2); BCD (n = 1); BCDH (n = 1) | 73 | 7 | 37 | 13 | 16 |
Hamrahlou | Humans/animals outdoors (n = 2) | 2 | – | – | 2 | – | – | 2 | – |
Khetay | Humans/animals outdoors (n = 15) | 15 | 15 | H (n = 8); D (n = 1); DH (n = 2); CD (n = 3); BCD (n = 1) | 15 | 4 | 5 | 2 | 4 |
Gungormaz | Humans/animals outdoors (n = 13) | 13 | 11 | H (n = 4); CD (n = 2); BCD (n = 1); BDH (n = 2); CDH (n = 2) | 13 | 4 | 4 | 3 | 2 |
Gholibaiglou | Humans/animals outdoors (n = 10) | 10 | 2 | D (n = 1); BDH (n = 1) | 10 | – | 4 | 6 | – |
Total | 113 | 46 | 46 | 113 | 15 | 50 | 26 | 22 | |