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Table 2 Identification of vertebrate hosts of the Simulium turgaicum complex based on the host cytb gene in ingested blood meals by multiplex and conventional PCR assays in the Aras River Basin, Iran

From: Molecular detection of Dirofilaria spp. and host blood-meal identification in the Simulium turgaicum complex (Diptera: Simuliidae) in the Aras River Basin, northwestern Iran

Village Ecotope (No. of blood-fed specimens) No. of blood-fed specimens in each village Multiplex PCR Host-specific PCR
n Hosts n Hosts
B H C D
Qarloujeh Humans/animals outdoors (n = 23); irrigation canals (n = 14); lands along the river (n =17); rice and alfalfa fields (n = 19) 73 18 DH (n = 1); CD (n = 2); BD (n = 2); CH (n = 9); BH (n = 2); BCD (n = 1); BCDH (n = 1) 73 7 37 13 16
Hamrahlou Humans/animals outdoors (n = 2) 2 2 2
Khetay Humans/animals outdoors (n = 15) 15 15 H (n = 8); D (n = 1); DH (n = 2); CD (n = 3); BCD (n = 1) 15 4 5 2 4
Gungormaz Humans/animals outdoors (n = 13) 13 11 H (n = 4); CD (n = 2); BCD (n = 1); BDH (n = 2); CDH (n = 2) 13 4 4 3 2
Gholibaiglou Humans/animals outdoors (n = 10) 10 2 D (n = 1); BDH (n = 1) 10 4 6
Total 113 46 46 113 15 50 26 22
  1. Abbreviations: B, bird; C, bovine; D, dog; H, human