Fig. 2
PCR verification, detection limit and specificity using outer primers F3 and B3 for genomic DNA amplification of Trichuris muris. a Detection limit of the expected 186 bp target length amplicon using PCR F3-B3. Lane M: molecular weight marker (100 bp Plus Blue DNA Ladder); Lane Tm: DNA of T. muris (20 ng); Lane N; negative control (ultrapure water, no DNA template). b Detection limit of PCR F3-B3. Lane M: molecular weight marker (100 bp Plus Blue DNA Ladder); Lane Tm: DNA of T. muris (20 ng); Lanes 10−1–10−10: 10-fold serial dilutions of T. muris DNA. c Specificity of PCR F3-B3. Lane M: 50 bp DNA ladder (Molecular weight marker XIII Roche); Lane Tm: DNA of T. muris (20 ng); Lanes Sm, Sh, Fh, Amp, Eg, Tsag, Tsol, Dd, Hd, Ans, Bp, Loa, Mp, Sv, As, Gd, Cp and Eh: DNA samples of Schistosoma mansoni, S. haematobium, Fasciola hepatica, Amphimerus sp., Echinococcus granulosus, Taenia saginata, T. solium, Dicrocoelium dendriticum, Hymenolepis diminuta, Anisakis simplex, Brugia pahangi, Loa loa, Mansonella perstans, Strongyloides venezuelensis, Ascaris suum, Giardia duodenalis, Cryptosporidium parvum and Entamoeba histolytica, respectively; Lane N: negative controls (ultrapure water, no DNA template)