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Table 1 Sequences of LAMP primers for the amplification of a partial consensus sequence based on the linear genomic DNA partial sequence in the 18S rDNA from Trichuris muris and T. trichiura

From: Whip-LAMP: a novel LAMP assay for the detection of Trichuris muris-derived DNA in stool and urine samples in a murine experimental infection model

Primer

Length (bp)

Sequence (5′–3′)

F3

20

ACTTTCGATGGTACGCTACG

B3

20

GCGTCTCATGGAGAATCGTT

FIP (F1c+F2)

40

TCTCAGGCTCCCTCTCCGGA-TGCTTACCATGGTGACAACG

BIP (B1c+B2)

41

GGCTACCACATCCAAGGAAGGC-GTCACTACCTCCCCGATCT

  1. Abbreviations: F3, forward outer primer; B3, reverse outer primer; FIP, forward inner primer (comprising F1c and F2 sequences); BIP, reverse inner primer (comprising B1c and B2 sequences)
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Parasites & Vectors

ISSN: 1756-3305

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