Table 2 Selected differentially expressed unigenes putatively involved in protein metabolism after blood-feeding and long-term starvation
Predicted Function | Annotation | TPM F-tick | TPM H-tick | TPM M-tick | ANOVA (P-value) | Unigene |
|---|---|---|---|---|---|---|
Proteinase | Midgut cysteine proteinase | 250.11 | 105.93 | 9.57 | 0.02 | DN42765 |
Hypothetical protein | 244.25 | 0.71 | 0.01 | 0 | DN53495 | |
Conserved hypothetical protein | 0 | 69.91 | 14.74 | 0 | DN35517 | |
Putative serine protease | 58.94 | 84.12 | 16.04 | 0 | DN46725 | |
Midgut cysteine proteinase 2 | 139.38 | 142.84 | 27.02 | 0 | DN40266 | |
Astricsin | 0 | 42.25 | 0 | 0 | DN38987 | |
Conserved hypothetical protein | 0.60 | 17.25 | 2.04 | 0 | DN56731 | |
Metalloprotease | Leucine aminopeptidase | 125.81 | 21.76 | 9.79 | 0 | DN46003 |
Conserved hypothetical protein | 233.58 | 60.91 | 36.54 | 0 | DN58308 | |
Astacin-like metallopeptidase | 10.37 | 2.76 | 0.60 | 0 | DN37813 | |
Neprilysin | 6.01 | 45.58 | 6.32 | 0 | DN54652 | |
Astacin-like metalloprotease | 8.29 | 0.05 | 0.04 | 0 | DN23285 | |
Membrane metallo-endopeptidase | 11.90 | 0.19 | 0.27 | 0 | DN54193 | |
Zinc metalloprotease | 6.17 | 26.69 | 2.60 | 0 | DN56686 | |
Cathepsin L | Cathepsin L-like cysteine proteinase | 441.24 | 427.17 | 84.50 | 0 | DN56942 |
Cathepsin L | 1,254.14 | 141.31 | 57.16 | 0 | DN40829 | |
Cathepsin B | Cathepsin B endopeptidase | 522.98 | 106.56 | 12.00 | 0 | DN54757 |
Cathepsin B | 65.82 | 46.24 | 11.38 | 0 | DN52552 | |
Cathepsin C | Cathepsin C precursor | 148.74 | 222.46 | 47.97 | 0 | DN42108 |
Cathepsin D | Yolk cathepsin | 138.57 | 0.05 | 0.04 | 0 | DN56458 |
Aspartic protease | 58.94 | 0.30 | 0.04 | 0 | DN33194 | |
Heme-binding aspartic proteinase | 146.34 | 0.11 | 0.05 | 0 | DN36019 | |
Aspartic protease | 35.25 | 127.92 | 10.05 | 0 | DN44103 | |
Carboxypeptidase | Carboxypeptidase | 3.50 | 0.08 | 23.44 | 0 | DN57960 |
Conserved hypothetical protein | 65.18 | 0.03 | 0.00 | 0 | DN54491 | |
Serine protease inhibitor | Serine protease inhibitor 3 | 199.21 | 9.24 | 16.00 | 0 | DN39339 |
Serine protease inhibitor 17 | 178.14 | 9.03 | 24.05 | 0 | DN54400 | |
Serine protease inhibitor 12 | 27.37 | 3.68 | 44.21 | 0 | DN53734 | |
Kunitz/Bovine pancreatic trypsin inhibitor Domain protein | 1,274.39 | 0.07 | 0.02 | 0 | DN56779 | |
Autophagy-related protein | Autophagy-related protein 16 | 3.39 | 9.07 | 3.64 | 0.04 | DN40035 |
Autophagy-related protein 9A | 7.65 | 9.52 | 6.28 | 0.32 | DN55372 | |
Autophagy-related protein 12 | 24.88 | 35.90 | 22.76 | 0.13 | DN37604 |