Molecular identification of native Wolbachia pipientis in Anopheles minimus in a low-malaria transmission area of Umphang Valley along the Thailand-Myanmar border

Table 1 Primers used in this study

Type	Targets	Primer names	Primer sequence (5ʹ–3ʹ)
Nested PCR	Wolbachia 16S rRNA	W-SpecF	CATACCTATTCGAAGGGATAG
		W-SpecR	AGCTTCGAGTGAAACCAATTC
		16SNF	GAAGGGATAGGGTCGGTTCG
		16SNR	CAATTCCCATGGCGTGACG
qPCR	Plasmodium 18S rRNA	PlasF	CTTAGTTACGATTAATAGGAGTAGC
	Plasmodium 18S rRNA	PlasR	GAAAATCTAAGAATTTCACCTCTGA
	Wolbachia 16S rRNA	W-SpecF	CATACCTATTCGAAGGGATAG
	Wolbachia 16S rRNA	W16S	TTGCGGGACTTAACCCAACA
	An. minimus ITS2A	ITS2A	TGT GAA CTG CAG GAC ACA T
	An. minimus ITS2A	MIA	CCC GTG CGA CTT GAC GA

ISSN: 1756-3305