Fig. 3

a Western blotting analysis to confirm the expression of the PfHRP2 protein. The supernatants of the parasite culture medium were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and a mouse monoclonal (MPFG-55P) antibody against Plasmodium falciparum (horseradish peroxidase [HRP]) was the primary antibody used for the Western blotting analysis to confirm HRP2 protein expression. HRP–goat anti-mouse was the secondary antibody. b Southern blotting analysis to confirm the disruption of Pfhrp2. The genomic DNA was digested overnight using the SacI restriction endonuclease. The DNAs were separated in an agarose gel and transferred to a membrane. The blots were hybridized with the labelled Pfhrp2 probe and exposed for 10 min. c Southern blotting analysis to confirm the presence of the drug resistance gene hDHFR. The genomic DNA was digested overnight using the PstI restriction endonuclease. The DNAs were separated in an agarose gel and transferred to a membrane. The blots were hybridized with the labelled hDHFR probe and exposed for 40 min. L2 Transgenic parasite, which was disrupted in only exon 2 of Pfhrp2