Fig. 2

Blocking of IL-25 induced alternative activation of macrophages after challenge infection. A Pattern of macrophage activation is different in primary and secondary infections analyzed according to the gene expression of marker mRNA of both classical (ArgII and iNOS) and alternative (ArgI and Ym-1) macrophage activation in the intestinal tissue of mα-IL-25-treated mice and non-treated mice after a challenge infection with E. caproni. B Expression of resistin-like molecule beta (RELM-β) mRNA in the intestinal tissue of mα-IL-25-treated ICR mice and non-treated mice after a challenge infection with E. caproni. The RQ of cytokine genes are shown after normalization with β-actin and standardization of the relative amount against the day 0 sample. Vertical bars represent the SD. Lowercase letters (a, b) above bars indicate either significant differences with respect to naïve mice controls (a) or significant differences between groups (b), at p  < 0.05