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Fig. 8 | Parasites & Vectors

Fig. 8

From: Identification and characterization of a Babesia bigemina thrombospondin-related superfamily member, TRAP-1: a novel antigen containing neutralizing epitopes involved in merozoite invasion

Fig. 8

Indirect immunofluorescence assay by confocal laser microscopy of B. bigemina with antibodies against recombinant BbiTRAP-1. Smears of B. bigemina-infected merozoites were incubated with sera from a bovine experimentally infected with B. bigemina (a–d), a control bovine immunized with adjuvant alone (eh), and a bovine immunized with recombinant BbiTRAP-1 (il). c, g, k Fluorescein isothiocyanate (FITC)-conjugated anti-bovine IgG and TO-PRO-3 DNA stain were used to create a merged image. Inset in i Enlargement of duplicated B. bigemina merozoites reacting with anti-BbiTRAP-1. DIC Differential interference contrast microscopy images are located on the far right and were used to confirm the location and number of individual intracellular parasites. Scale bars: 5 μm

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