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Table 1 Dengue virus (DENV)-infected mosquito samples validated by reverse transcription-polymerase chain reaction (RT-PCR) amplification and sequencing of the partial sequence of the DENV E gene

From: Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines

Sample code Barangaya Household category Total RNA concentration (ng/μl) Mean Ct value Detection Validation Mosquito DENV serotype Patient DENV serotype
One-step multiplex real-time RT-PCR RT-PCR of partial E gene Sequencing of partial E gene
SI5-5 San Isidro 3 43.4 23.46 + + + DENV-1
AS2-2 San Miguel 1 131.1 23.26 + + + DENV-2 DENV-1/DENV-3
SI1-1 San Isidro 2 83.0 15.40 + + + DENV-2
SI6-2 San Isidro 3 8.1 34.66 +
SI6-3 San Isidro 3 77.1 20.69 + + + DENV-2
SI4-4 San Isidro 3 107.5 34.76 +
SB6-6 San Sebastian 3 36.6 35.97 +
SB4-22 San Sebastian 3 7.8 35.47 + + + DENV-2
SB4-12 San Sebastian 3 36.3 34.82 + + + DENV-2
SB4-53 San Sebastian 3 113.6 31.23 + + + DENV-2
SB3-30 San Sebastian 3 8.5 36.11 +
AS10-49 Maliwalo 1 32.6 29.14 + + + DENV-4 DENV-1
AS10-29 Maliwalo 1 106.7 26.36 + + + DENV-4 DENV-1
AS12-4 Dalayap 1 122.8 36.20 + + + DENV-4
  1. Ct Cycle threshold
  2. aAdministrative unit comprising 50–100 families