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Table 1 Dengue virus (DENV)-infected mosquito samples validated by reverse transcription-polymerase chain reaction (RT-PCR) amplification and sequencing of the partial sequence of the DENV E gene

From: Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines

Sample code

Barangaya

Household category

Total RNA concentration (ng/μl)

Mean Ct value

Detection

Validation

Mosquito DENV serotype

Patient DENV serotype

One-step multiplex real-time RT-PCR

RT-PCR of partial E gene

Sequencing of partial E gene

SI5-5

San Isidro

3

43.4

23.46

+

+

+

DENV-1

–

AS2-2

San Miguel

1

131.1

23.26

+

+

+

DENV-2

DENV-1/DENV-3

SI1-1

San Isidro

2

83.0

15.40

+

+

+

DENV-2

–

SI6-2

San Isidro

3

8.1

34.66

+

–

–

–

–

SI6-3

San Isidro

3

77.1

20.69

+

+

+

DENV-2

–

SI4-4

San Isidro

3

107.5

34.76

+

–

–

–

–

SB6-6

San Sebastian

3

36.6

35.97

+

–

–

–

–

SB4-22

San Sebastian

3

7.8

35.47

+

+

+

DENV-2

–

SB4-12

San Sebastian

3

36.3

34.82

+

+

+

DENV-2

–

SB4-53

San Sebastian

3

113.6

31.23

+

+

+

DENV-2

–

SB3-30

San Sebastian

3

8.5

36.11

+

–

–

–

–

AS10-49

Maliwalo

1

32.6

29.14

+

+

+

DENV-4

DENV-1

AS10-29

Maliwalo

1

106.7

26.36

+

+

+

DENV-4

DENV-1

AS12-4

Dalayap

1

122.8

36.20

+

+

+

DENV-4

–

  1. Ct Cycle threshold
  2. aAdministrative unit comprising 50–100 families