Table 2 Molecular examination of Sarcocystis species from cattle based on cox1 sequence analysis
Sarcocystis species | Sample type | Cox1 GenBank accession no. (length in bp) | Differences between obtained sequences (%) | Sequence identity (%) | |
|---|---|---|---|---|---|
Comparison of isolates of the same species | Comparison of isolates with other closely related species | ||||
S. bovifelis | Sarcocyst | MT796903–MT796925 (1038) | 0–1.0 | 98.4–100% to S. bovifelis (KT900961–KT900998, KC209690–KC209696, MK962347–MK962348) | 93.0–93.9% to S. bovini (KT900999–KT901022, LC171858) |
Digested | MT796952–MT796954 (471) | 0 | 97.9–100% to S. bovifelis | 92.4–93.0% to S. bovini | |
S. cruzi | Sarcocyst | MT796926–MT796945 (1038) | 0.2–1.2 | 96.1–99.9% to S. cruzi (KC209597–KC209600, KT901078–KT901095, LC171859–LC171862, MG787071–MG787076) | 92.6–93.9 to S. levinei (KU247874–KU247885, MH255771–MH255781) |
Digested | MT796955–MT796957 (478) | 0–0.4 | 97.7–99.8% to S. cruzi | < 95% similarity with other Sarcocystis spp. | |
S. hirsuta | Sarcocyst | MT796946–MT796951 (1038) | 0–0.7 | 99.2–100% to S. hirsuta (KC209634, KT901023–KT901077, LC171863) | 93.4–93.7 to S. buffalonis (KU247868–KU247873, MG792800–MG792802) |
Digested | MT796958–MT796960 (461) | 0–0.2 | 99.4–100% to S. hirsuta | 95.6–96.3% to S. buffalonis | |
S. hominis | Digested | MT796961–MT796964 (501) | 0 | 98.2–98.6% to S. hominis (MH021119, MK497840–MK497843) | < 90% similarity with other Sarcocystis spp. |