Fig. 4
From: Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3
Zymography showed low hydrolytic activity of AcCP3 gene knockdown trophozoites. a AcCP3 mRNA expression level of wild-type trophozoites (PYG); only superfect-treated trophozoites (superfectin); negative siRNA transfected trophozoites; AcCP3-siRNA transfected trophozoites. b Zymography test of different trophozoite crude extract proteins. Lane 1 and lane 4 showed normal trophozoite crudes proteins with no treatment. Lane 5 and lane 10 showed normal trophozoite crude proteins pretreated with 40 mM NEM. Lane 9 added negative siRNA transfected trophozoite crude proteins. Lane 11 added AcCP3 gene knockdown trophozoite crude proteins. Independent experiments were repeated three times. c The optic densities of the zymography test. Significance was calculated by one-way analysis of variance (ANOVA) followed by a Tukey test. Vertical bars indicate SD. ***P < 0.001