Fig. 5
From: Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3
Microscopy and CCK-8 demonstrating that AcCP3 gene knockdown trophozoites exhibited a decrease in Acanthamoeba-mediated HCEC cytotoxicity. a–d Pictures taken under light microscopy. a Normal cultured confluent HCEC (treat 1). b HCEC cocultured with normal trophozoites (treat 2). c HCEC cocultured with negative siRNA transfected trophozoites (treat 3). d HCEC cocultured with AcCP3-knockdown trophozoites (treat 4). e Percentage of cell coverage to the basal area. f OD 450-nm-fold decrease compared to treat 1 HCEC cell. Independent experiments were repeated three times. Vertical bars indicate SD. *P < 0.05; **P < 0.01; ***P < 0.001