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Fig. 6 | Parasites & Vectors

Fig. 6

From: Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3

Fig. 6

AcCP3-siRNA significantly decreased the phosphorylation levels of Raf, Erk and p53 in Acanthamoeba-mediated HCEC cells. Four groups of HCEC were treated the same as above. a qRT-PCR analyzed the mRNA expression level of K-Ras gene. b Western blot analysis of the phosphorylation level of Raf, Erk and p53 proteins in HCEC cells. c The optic densities of phosphorylation levels of Raf, Erk and p53 proteins in HCEC cells. Normal cultured confluent HCEC (treat 1); HCEC cocultured with normal trophozoites (treat 2); HCEC cocultured with negative siRNA transfected trophozoites (treat 3); HCEC cocultured with AcCP3-knockdown trophozoites (treat 4). Independent experiments were repeated three times. Vertical bars indicate SD. *P < 0.05; **P < 0.01; ***P < 0.001

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