Table 2 Primers used for piroplasm parasite detection
Primer’s target region | Primer name | Primer sequence (5′–3′) | References |
|---|---|---|---|
18S rRNA V4 hyper-variable region | Reverse Line Blot-F (RLB-F) | GAGGTAGTGACAAGAAATAACAATA | [24] |
Reverse Line Blot-R (RLB-R) | TCTTCGATCCCCTAACTTTC | ||
Illumine tail-tagged RLB primer | Illumina RLB-F | ACACTCTTTCCCTACACGACGCTCTTCCGATCT[RLB-F] | Â |
Illumina RLB-R | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT[RLB-R] | ||
Illumina-index primer | Illumina-i5 primers | AATGATACGGCGACCACCGAGATCTACAC[index*] ACACTCTTTCCCTACACGACGCTCTTCCGATCT | Â |
Illumina-i7 primers | CAAGCAGAAGACGGCATACGAGAT[index*] GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |