Fig. 1

Alignment of 27 L. cuprina SCP/TAPS. The 27 SCP/TAPS identified in L. cuprina were aligned using MUSCLE (47) and manually filtered to remove alignment positions with > 50% gap frequency. Conserved cysteine residues are highlighted in yellow and indicated by an arrow. Black dots represent conserved residues common to the CAP domain. Red bars below the sequence alignment indicate CAP domain location. The sequence logo indicates the prevalence of an amino acid at each position based on the size of the letter, and numbers indicate the amino acid position in the alignment. Residues that are highly conserved (≥ 90%) are highlighted based on biochemical properties (see Geneious version 7.1.9). Regions with low (< 30%) similarity (such as the proximal CAP domain of LCSc0-DD1) are not displayed here