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Fig 6. | Parasites & Vectors

Fig 6.

From: Developmental regulation of Dirofilaria immitis microfilariae and evaluation of ecdysone signaling pathway transcript level using droplet digital PCR

Fig 6.

Transcript level of the ecdysone signaling system in D. immitis using droplet digital PCR. Fold changes for both no-cell and cell-conditioned media groups were calculated relative to day 1 of each medium. Fold changes for adults were calculated relative to day 1 of the cell-conditioned media group. Insert figures show transcript level at day 1 for both media. All transcript levels were normalized to three reference genes: Dim-GAPDH, Dim-Actin, and Dim-β-tubulin. a Dim-EcR, b Dim-rxr-1, c Dim-nhr-6, d Dim-nhr-7. All data are reported as mean fold change, with error bars corresponding to the SD from at least two individual replicates that were performed three times. The significance of the transcript level fold changes compared to day 1 baseline level was analyzed by the unpaired t-test with Welch’s correction, two-tailed p value, and 95% confidence interval using Prism 6.0c (Graph Pad Software, Inc.). Asterisks denote the statistical difference between no-cell microfilariae (red asterisks), cell-conditioned media microfilariae (blue asterisks), adults (green asterisks) vs day 1 groups (---). *p < 0.05, **p < 0.01, ***p < 0.001

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