Skip to main content
Fig 7. | Parasites & Vectors

Fig 7.

From: Developmental regulation of Dirofilaria immitis microfilariae and evaluation of ecdysone signaling pathway transcript level using droplet digital PCR

Fig 7.

Development of D. immitis microfilariae treated with 20-hydroxyecdysone (HP-β-CD). Results show percentages of larvae at the microfilaria, pre-sausage, sausage, and late sausage stages cultured in 0.05% DMSO + 0.004% HP-β-CD (a), 5 µM 20E + 0.05% DMSO + 0.004% HP-β-CD (b). All data are reported as mean percentages, with error bars corresponding to the SD from at least three individual replicates. The significance of the effects of 20E on D. immitis development was analyzed by the Mann-Whitney U-test, two-tailed P value, and 95% confidence interval using Prism 6.0c (Graph Pad Software, Inc.). Asterisks denote the statistical difference between total (red asterisks) and healthy (blue asterisks) parasites vs DMSO + HP-β-CD group. *p < 0.05, **p < 0.01, ***p < 0.001. c Proportion of motile D. immitis larvae cultured in 0.05% DMSO + 0.004% HP-β-CD or 5 µM 20E + 0.05% DMSO + 0.004% HP-β-CD. The significance of the effects of 20E on D. immitis the proportion of larvae that were motile was analyzed by the Mann-Whitney U-test, two-tailed P value, and 95% confidence interval using Prism 6.0c (Graph Pad Software, Inc.). Asterisks denote the statistical difference between 5 µM 20E + DMSO + HP-β-CD vs DMSO + HP-β-CD group. *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page