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Table 3 Effect of ammonia, water types, and feed regimes on life history stages (Experiment 1)

From: Laboratory and microcosm experiments reveal contrasted adaptive responses to ammonia and water mineralisation in aquatic stages of the sibling species Anopheles gambiae (sensu stricto) and Anopheles coluzzii

Species

Water type

Feed regime

Ammonia (mg/l)

% Larval survival

% Pupal mortality

% Adult emergence

An. coluzzii

Deionised

Solution

0

77 (59–88)

10 (3–26)

67 (49–81)

0.6

73 (56–86)

0

73 (56–86)

1.3

70 (52–83)

13 (5–30)

57 (39–73)

2.5

57 (39–73)

3 (1–17)

53 (36–70)

12.5

33 (19–51)

23 (12–41)

10 (3–26)

25

3 (0–17)

3 (0–17)

0

62.5

0

0

0

Powder

0

77 (59–88)

3 (1–17)

73 (56–86)

0.6

83 (66–93)

27 (14–44)

57 (39–73)

1.3

80 (63–90)

0

80 (63–90)

2.5

50 (33–67)

3 (1–17)

47 (30–64)

12.5

0

0

0

25

0

0

0

62.5

0

0

0

Mineral

Solution

0

80 (63–90)

7 (2–21)

73 (56–86)

0.6

80 (63–90)

3 (1–17)

77 (59–88)

1.3

73 (56–86)

10 (3–26)

63 (46–78)

2.5

67 (49–81)

3 (1–17)

63 (46–78)

12.5

73 (56–86)

3 (1–17)

70 (52–83)

25

10 (3–26)

3 (1–17)

7 (2–21)

62.5

0

0

0

Powder

0

83 (66–93)

10 (3–26)

73 (56–86)

0.6

80 (63–90)

3 (1–17)

77 (59–88)

1.3

60 (42–75)

3 (1–17)

57 (39–73)

2.5

73 (56–86)

7 (2–21)

67 (49–81)

12.5

70 (52–83)

17 (7–34)

53 (36–70)

25

0

0

0

62.5

0

0

0

An. gambiae (s.s.)

Deionised

Solution

0

86 (70–95)

7 (2–21)

80 (63–90)

0.6

97 (83–99)

10 (3–26)

87 (70–95)

1.3

90 (74–97)

0

90 (74–97)

2.5

87 (70–95)

17 (13–34)

70 (52–83)

12.5

43 (27–61)

23 (12–41)

20 (10–37)

25

0

0

0

62.5

0

0

0

Powder

0

93 (79–98)

 

93 (79–98)

0.6

87 (70–95)

13 (5–30)

73 (56–86)

1.3

93 (79–98)

27 (14–44)

67 (49–81)

2.5

100(89–100)

47 (30–64)

53 (36–70)

12.5

13 (5–30)

13 (5–30)

0

25

0

0

0

62.5

0

0

0

Mineral

Solution

0

93 (79–98)

17 (7–34)

77 (59–88)

0.6

97 (83–99) 30

10 (3–26) 30

87 (70–95) 30

1.3

87 (70–95)

3 (1–17)

83 (66–93)

2.5

100(89–100)

0

100(89–100)

12.5

93 (79–98)

10 (3–26)

83 (66–93)

25

13 (5–30)

7 (2–21)

7 (2–21)

62.5

0

0

0

Powder

0

93 (79–98)

7 (2–21)

87 (70–95)

0.6

90 (74–97)

3 (1–7)

87 (70–95)

1.3

97 (83–99)

0

97 (83–99)

2.5

93 (79–98)

10 (3–26)

83 (66–93)

12.5

90 (74–97)

13 (5–30)

77 (59–88)

25

20 (10–37)

20 (10–37)

0

62.5

0

0

0

  1. Ninety-five percent confidence intervals are in parentheses. Larval survival, pupal mortality, and emergence rates were calculated out of an initial sample size of 30 larvae (per treatment)