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Table 2 Bioinformatic analysis data for each step in processing of MinION sequences obtained from the specimens used in this study

From: Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene

Specimen ID

1

2

3

4

5

6

7

Total MinION reads

260,471

272,100

574,327

562,189

509,070

505,177

335,913

Total Mbases

458.1

510

887.2

547.9

657.2

474.4

259.5

Reads > Q7

236,039

247,399

515,724

529,182

431,051

456,117

296,619

Mbases > Q7

428.1

479

815.5

524.3

574.4

434.6

233

Reads with length 1000–2100 nt

93,828

89,141

131,127

105,440

109,609

87,019

55,970

Reads after canu correction

79,682

61,484

77,149

69,586

57,435

50,107

28,966

Reads after canu trimming

71,895

55,643

46,519

44,990

29,693

27,873

19,805

Strand (+) reads with both forward and reverse primers

15,730

9,573

7,310

8,734

4,620

3,430

1,376

Strand (−) reads with both forward and reverse primers

14,242

9,407

5,646

6,427

3,816

2,616

986

Strand (+) and (−) reads combined

29,972

18,980

12,956

15,161

8,436

6,046

2,362

Reads that aligned to a Blastocystis reference with > 90% identity and coverage

26,314

17,960

4,626

2,581

4,309

1,077

287

No. clusters after clustering reads at 98% identity

34

4,556

265

256

682

152

47

No. clusters with an abundance > 5

18

25

12

7

11

7

3

Total abundance of all clusters that had at least 5 reads

26,116

10,674

4,353

2,158

3,585

725

30

No. clusters that aligned to expected Blastocystis reference after racon polishing and re-clustering

1

1

1

2

1

2

1

No. clusters after nanopolishing and re-clustering

1

1

1

2

1

2

1