Specimen ID | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
---|---|---|---|---|---|---|---|
Total MinION reads | 260,471 | 272,100 | 574,327 | 562,189 | 509,070 | 505,177 | 335,913 |
Total Mbases | 458.1 | 510 | 887.2 | 547.9 | 657.2 | 474.4 | 259.5 |
Reads > Q7 | 236,039 | 247,399 | 515,724 | 529,182 | 431,051 | 456,117 | 296,619 |
Mbases > Q7 | 428.1 | 479 | 815.5 | 524.3 | 574.4 | 434.6 | 233 |
Reads with length 1000–2100 nt | 93,828 | 89,141 | 131,127 | 105,440 | 109,609 | 87,019 | 55,970 |
Reads after canu correction | 79,682 | 61,484 | 77,149 | 69,586 | 57,435 | 50,107 | 28,966 |
Reads after canu trimming | 71,895 | 55,643 | 46,519 | 44,990 | 29,693 | 27,873 | 19,805 |
Strand (+) reads with both forward and reverse primers | 15,730 | 9,573 | 7,310 | 8,734 | 4,620 | 3,430 | 1,376 |
Strand (−) reads with both forward and reverse primers | 14,242 | 9,407 | 5,646 | 6,427 | 3,816 | 2,616 | 986 |
Strand (+) and (−) reads combined | 29,972 | 18,980 | 12,956 | 15,161 | 8,436 | 6,046 | 2,362 |
Reads that aligned to a Blastocystis reference with > 90% identity and coverage | 26,314 | 17,960 | 4,626 | 2,581 | 4,309 | 1,077 | 287 |
No. clusters after clustering reads at 98% identity | 34 | 4,556 | 265 | 256 | 682 | 152 | 47 |
No. clusters with an abundance > 5 | 18 | 25 | 12 | 7 | 11 | 7 | 3 |
Total abundance of all clusters that had at least 5 reads | 26,116 | 10,674 | 4,353 | 2,158 | 3,585 | 725 | 30 |
No. clusters that aligned to expected Blastocystis reference after racon polishing and re-clustering | 1 | 1 | 1 | 2 | 1 | 2 | 1 |
No. clusters after nanopolishing and re-clustering | 1 | 1 | 1 | 2 | 1 | 2 | 1 |