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Table 2 Bioinformatic analysis data for each step in processing of MinION sequences obtained from the specimens used in this study

From: Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene

Specimen ID 1 2 3 4 5 6 7
Total MinION reads 260,471 272,100 574,327 562,189 509,070 505,177 335,913
Total Mbases 458.1 510 887.2 547.9 657.2 474.4 259.5
Reads > Q7 236,039 247,399 515,724 529,182 431,051 456,117 296,619
Mbases > Q7 428.1 479 815.5 524.3 574.4 434.6 233
Reads with length 1000–2100 nt 93,828 89,141 131,127 105,440 109,609 87,019 55,970
Reads after canu correction 79,682 61,484 77,149 69,586 57,435 50,107 28,966
Reads after canu trimming 71,895 55,643 46,519 44,990 29,693 27,873 19,805
Strand (+) reads with both forward and reverse primers 15,730 9,573 7,310 8,734 4,620 3,430 1,376
Strand (−) reads with both forward and reverse primers 14,242 9,407 5,646 6,427 3,816 2,616 986
Strand (+) and (−) reads combined 29,972 18,980 12,956 15,161 8,436 6,046 2,362
Reads that aligned to a Blastocystis reference with > 90% identity and coverage 26,314 17,960 4,626 2,581 4,309 1,077 287
No. clusters after clustering reads at 98% identity 34 4,556 265 256 682 152 47
No. clusters with an abundance > 5 18 25 12 7 11 7 3
Total abundance of all clusters that had at least 5 reads 26,116 10,674 4,353 2,158 3,585 725 30
No. clusters that aligned to expected Blastocystis reference after racon polishing and re-clustering 1 1 1 2 1 2 1
No. clusters after nanopolishing and re-clustering 1 1 1 2 1 2 1