Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene

Table 3 Comparison of full-length Blastocystis SSU rRNA gene sequences generated in this study by MinION sequencing to Illumina MiSeq sequences from the same sample and closest full-length match available on GenBank

Specimen ID	Blastocystis subtype	Length of sequence generated by MinION in this study (GenBank accession number)	Similarity to Illumina MiSeq sequence^a (%)	Similarity to closest match available in GenBank (sequence length, accession number)
1	ST1	1766 bp (MT898451)	100	99.8 (1770 bp, U51151)
2	ST4	1772 bp (MT898452)	100	100 (1730 bp, AY590114)
3	ST4	1773 bp (MT898453)	100	99.9 (1730 bp, AY590114)
4	ST11	1762 bp (MT898454)	100	99.9 (989 bp, GU256903)
4	ST11	1763 bp (MT898455)	100	98.6 (989 bp, GU256929)
5	ST10	1770 bp (MT898456)	99.8	99.5 (1728 bp, KC148207)
6	ST10	1770 bp (MT898457)	99.8	99.5 (1728 bp, KC148207)
6	ST14	1771 bp (MT898458)	99.8	99.6 (1772 bp, KC148205)
7	ST14	1771 bp (MT898459)	99.8	99.7 (1772 bp, KC148205)

^aIllumina MiSeq sequence corresponds to the approximately 480 bp region of the SSU rRNA gene amplified using the primers reported in [11] using methods reported in [12]

ISSN: 1756-3305