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Table 3 Comparison of full-length Blastocystis SSU rRNA gene sequences generated in this study by MinION sequencing to Illumina MiSeq sequences from the same sample and closest full-length match available on GenBank

From: Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene

Specimen ID Blastocystis subtype Length of sequence generated by MinION in this study (GenBank accession number) Similarity to Illumina MiSeq sequencea (%) Similarity to closest match available in GenBank (sequence length, accession number)
1 ST1 1766 bp (MT898451) 100 99.8 (1770 bp, U51151)
2 ST4 1772 bp (MT898452) 100 100 (1730 bp, AY590114)
3 ST4 1773 bp (MT898453) 100 99.9 (1730 bp, AY590114)
4 ST11 1762 bp (MT898454) 100 99.9 (989 bp, GU256903)
1763 bp (MT898455) 100 98.6 (989 bp, GU256929)
5 ST10 1770 bp (MT898456) 99.8 99.5 (1728 bp, KC148207)
6 ST10 1770 bp (MT898457) 99.8 99.5 (1728 bp, KC148207)
ST14 1771 bp (MT898458) 99.8 99.6 (1772 bp, KC148205)
7 ST14 1771 bp (MT898459) 99.8 99.7 (1772 bp, KC148205)
  1. aIllumina MiSeq sequence corresponds to the approximately 480 bp region of the SSU rRNA gene amplified using the primers reported in [11] using methods reported in [12]