Fig. 1
Cq values for Culicoides sonorensis infected with bovine ephemeral fever virus. Midges were orally fed a blood–virus suspension (oral; red) and incubated for 8 days or intrathoracically inoculated (IT) with virus and tested after incubation for 5 (IT, day 5; blue) or 6 days (IT, day 6; magenta) or after 6–7 days incubation and feeding on naïve calves (IT, fed on cattle; cyan). Individuals were processed as whole insects (diamonds) or dissected heads (downward-pointing triangles) or bodies (upward-pointing triangles). Black lines indicate the median value, excluding insects with no Cq value