Table 1 Primers developed to detect bovine ephemeral fever virus
Primer | Sequence | Final concentration | Genome location (BEFV genome MN078236) |
|---|---|---|---|
BEFV_F | 5'-CATTGGGAATGCATTACAGT-3' | 10 pmoles/ml | 3683–3202 |
BEFV_P | 5'-[6FAM]AGATTATGGGAAGCTCCAGA[TAM ]-3' | 5 pmoles/µl | 3737–3756 |
BEFV_R | 5'-GTTTGGTTTTCTATACTCCAC-3' | 10 pmoles/µl | 3838–3858 |