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Fig. 1 | Parasites & Vectors

Fig. 1

From: Downregulated RPS-30 in Angiostrongylus cantonensis L5 plays a defensive role against damage due to oxidative stress

Fig. 1

Cloning strategy for the reporter and rescuing constructs. a Sequences upstream of Acan-rps-30 5′-UTRs, about 2000 bp, was used as putative promoters. The promoter regions of Acan-rps-30 and Ce-rps-30, fused with green fluorescent protein (GFP) downstream, were cloned into plasmid pPD95.77 to construct pAcan-rps-30::gfp and pCe-rps30::gfp, respectively. bThe Acan-rps-30 cDNA sequence, fused with red fluorescent protein (RFP) downstream was cloned into plasmid pPD95.77, using pCe-rps30 as promoter, to construct pCe-rps-30::Acan-rps-30::rfp. Acan-RPS-30 Angiostrongylus cantonensis ribosomal protein 30, UTR Untranslated region

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